Chemical comparison between stems and leaves of Cupressus sempervirens l. ("Cypress") of Cuban origin, as anti-inflammatories

Abstract

Cupressus sempervirens Linnaeus (Cypress), belonging to the botanical family Cupressaceae, is of great importance for presenting metabolites with great therapeutic potential; We set the objective of comparing the chemical characterization of the Cuban ¨ Cupressus sempervirens L. (Cypress) ¨ in stems and leaves; as anti-inflammatory. Quality parameters of the leaves and stem were evaluated: organoleptic characteristics and by Near Infrared residual humidity, total ash, protein, fiber and starch, most of the results obtained comply with the specifications described in the official monographs, The extraction method carried out was Maceration, using 95% ethanol as menstruum. For the physical-chemical analysis of the extract, the following parameters were determined: organoleptic characteristics, relative density, refractive index, pH and total solids. The qualitative chemical composition of the plant was developed through Phytochemical Screening; expressing: essential oils, fats, alkaloids, triterpenoids and steroids, resins, reducing sugars, saponins, flavonoids, phenolic compounds, mucilages and bitter principles and by high resolution liquid chromatography; suggests that possibly the compounds that have a retention time in stems of 15,2 and 15,9 minutes and those with a retention time in leaves of 15,2 and 16.0 minutes, respectively, are two compounds that represent the same type of metabolites, in each case, produced by the plant in each part of the plant; separately. Compounds present in leaves that do not appear in stems and vice versa are shown. The anti-inflammatory activity was determined by the Auricular Edema Method of the ethanolic extract, which showed the expected result for the species.

Keywords

Cupressus sempervirens L., chemical composition, PINACEAES

References

1. Aligiannis, N., E. Kalpoutzakis, S. Mitaku, I.B. Chinou (2001): Composition and antimicrobial activity of the essential oils of two Origanum species. J. Agric Food Chem 49(9): 2900-2920, 4168-70.
2. Avello, M y.Cisternas, I. (2010): Fitoterapia, sus orígenes, características y situación en Chile. RevMed Chile. 138: 1288-1293
3. Cáceres A. (1999). Plantas de uso medicinal en Guatemala, Editorial Universitaria, Ciudad de Guatemala, Guatemala. 1999.
4. Cañigueral S, Vila R. (2005). La Fitoterapia como herramienta terapéutica. Ginecología y Obstetricia Clínica 2005; 6(1):43-51
5. Castro, I. (2009). Actualidad de la Medicina Tradicional Herbolaria. Editorial. Rev. CubPlantMed. 11 (2)
6. Chaudhary HJ, Shahid W, Bano A, Ullah F, Munis F, Fahad S and Ahmad I. (2012). In vitro analysis of Cupressus sempervirens L plant extracts antibaterial activity. Journal of Medicinal Plants Research 2012; 6(2): 273-276.
7. Christenhusz, M. J. M., J. L. Reveal, A. K. Farjon, M. F. Gardner, R. R. Mill & M. W. Chase. (2011). A new classification and linear sequence of extant gymnosperms. Phytotaxa 19: 55–70. Referencias Bibliográficas 2017
8. CONABIO. (2009). Catálogo taxonómico de especies de México. 1. In Capital Nat. México. CONABIO, Mexico City
9. CYTED. (1999). Curso para Investigadores en el descubrimiento de nuevos medicamentos, Lima Noviembre de 1996, Edema Auricular pp 83.
10. Esau, K. (1985). Anatomía Vegetal. 3a Ed. rev. Barcelona.
11. Gómez Estrada, Harold Alberto; González Ruiz, Karina Noreica; Medina Domingo, José. Actividad Antiinflamatoria de Productos Naturales Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, vol. 10, núm. 3, mayo, 2011, pp. 182-217.
12. Guide fortheCare and Use of Laboratory Animals, 1987. National Research Council. Washintong, DC: The National Academies Press.
13. Laubenfels, D. J., C. E. Husby& P. Griffith. 2012. Further nomenclatural action for the cypressus (Cupressaceae). Novon 22(1): 8–15 Referencias Bibliográficas 2017
14. Miranda, M. y Cuellar, A. (2000). Manual de prácticas de laboratorio de análisis farmacognóstico. Félix Varela. 2000.
15. Miranda, M. y Cuéllar, A. (2001): Farmacognosia y Productos Naturales. Libro de texto. Félix Varela.
16. Miranda, M. y Cuéllar, A. (2001). Farmacognosia y Química de Productos Naturales. Editorial Félix Varela. 2da Edición.
17. Universidad autónoma de Madrid-https://repositorio.uam.es>handle
18. Fuente, V. R. Revista UH. https://revistas.uh.cu>article. PDF
19. Universidad de Oviedo-https://digibuo..uniovi.es>T...PDF.
20. Gale-https://go.gale.com>i.do.Propiedadesmedicinalesdeplantasconacytpyhttps://www.conacyt.gov.py>PDF
21. Scribd-https://es.scribd.com>document PDF-Cromatografia líquida de alto rendimiento
22. Moya, B., & Moya, J. (2013). El" sistema ciprés" de barreras cortafuegos: selvicultura preventiva.
23. Organización Mundial de la Salud (OMS). Informe sobre la salud en el mundo 1998: la vida en el siglo XXI: una perspectiva para todos: resumen.
24. Roig, JT. (1988). Plantas medicinales, aromáticas o venenosas de Cuba. La Habana. Ed. Científico-Técnica, 1125.
25. Voegel, G. (1997). Drugs Discovery
26. Waller, G.R y E.K. Nowacki (1978): Role of alkaloids. Alkaloid Biology and Metabolism in Plants. Plenus Press, New York. 249pp

How to Cite

Download Citation

• Endnote/Zotero/Mendeley (RIS)
• BibTeX

This work is licensed under a Creative Commons Attribution 4.0 International License.